An area within the laboratory environment of level 2 of Westmead Hospital was gutted, the existing services redirected where necessary then partitions and suspended ceiling installed, hermetically sealed so that pressurized environments could be created as a part of cross infection protections. The ceiling space was made trafficable to cater for the air conditioning, filter system, lighting maintenance etc. with partitions and doorways below to separate the various parts of this PC2 laboratory with its pressure graded cross infection control entry and exit chambers (that also functioned as gowning area and secondary laboratory respectively) and the “Open Flow” laboratory, and “Cryopreservation” room.
The rooms and all lights and power outlets were hermetically sealed and sophisticated air conditioning with HEPA Filters installed to the “Sterile Suite” and its entry and exit chambers capable of maintaining working temperatures of 16 degrees Celsius and particulate ingress at less than 35 parts per million in the Bone Marrow Laboratory itself. This and the new systems to the rest of the rooms were balanced to maintain 30pa of pressure differentiation from the Sterile Suite to the ancillary rooms to avoid contamination back through the entry and exit chambers.
Sophisticated alarms were installed to warn of oxygen depletion, pressurization failure, temperature excess and equipment (fridges etc) malfunction, many of these with remote links back to the Blood Bank in the Hospital and the Building Maintenance Control System. Fan speeds for pressurization and thermostats for temperature were controllable from within the Sterile Suite. This is regarded as one of the most sophisticated laboratories of its kind in Australia and worldwide and now processes the bone marrow for a large part of our Public and Private Health network.